Listen In - Bitesize Bio Webinar Audios

Bitesize Bio

The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever. Each episode is an opportunity to gain the valuable insights you need to advance your research. From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much more—you'll hear about challenges encountered and discover practical solutions to achieve the best possible results. Tap into the experience and expertise of leading researchers and commercial specialists to drive your research projects forward efficiently and productively. Listen In now! https://bitesizebio.com/listen-in/ read less

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Multi-View Light Sheet Microscopy to Track Cell Lineages in Tissue Morphogenesis

Multi-View Light Sheet Microscopy to Track Cell Lineages in Tissue Morphogenesis

A major question in developmental biology is understanding how cellular fate decisions are regulated precisely in space and time. Fortunately, we can now begin to observe these processes with modern imaging approaches, which allow the tracking of cell fate decision events and cellular rearrangements by live 3D time-lapse microscopy. This development is shifting our understanding of pattern formation in development away from static models towards models based on the principles of dynamical systems and statistical mechanics. In this episode of Listen In, hear how light-sheet microscopy is the technique of choice for volumetric live imaging of 3D samples at high speed with minimal phototoxicity. See two user examples of how light-sheet microscopy is helping scientists understand the biological process of intestinal and brain organoid development.We also explore the technology behind the Leica Viventis LS2 Live, which combines dual-view illumination and detection in an open-top configuration. You will also learn how this unique configuration allows easy sample mounting and long-term imaging from small embryos and organoids to large multicellular systems. Watch the full presentation here: https://microscopyfocus.com/multi-view-light-sheet-microscopy-to-track-cell-lineages-in-tissue-morphogenesis/"Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Episodes

Multi-View Light Sheet Microscopy to Track Cell Lineages in Tissue Morphogenesis

Multi-View Light Sheet Microscopy to Track Cell Lineages in Tissue Morphogenesis

A major question in developmental biology is understanding how cellular fate decisions are regulated precisely in space and time. Fortunately, we can now begin to observe these processes with modern imaging approaches, which allow the tracking of cell fate decision events and cellular rearrangements by live 3D time-lapse microscopy. This development is shifting our understanding of pattern formation in development away from static models towards models based on the principles of dynamical systems and statistical mechanics. In this episode of Listen In, hear how light-sheet microscopy is the technique of choice for volumetric live imaging of 3D samples at high speed with minimal phototoxicity. See two user examples of how light-sheet microscopy is helping scientists understand the biological process of intestinal and brain organoid development.We also explore the technology behind the Leica Viventis LS2 Live, which combines dual-view illumination and detection in an open-top configuration. You will also learn how this unique configuration allows easy sample mounting and long-term imaging from small embryos and organoids to large multicellular systems. Watch the full presentation here: https://microscopyfocus.com/multi-view-light-sheet-microscopy-to-track-cell-lineages-in-tissue-morphogenesis/"Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Enhancing 3D Spatial Biology with AI: Simplified Insights for All

Enhancing 3D Spatial Biology with AI: Simplified Insights for All

In this episode, learn how AI-powered segmentation, spatial analysis, and phenotyping can help you gain new insights for 3D images with complex morphological measurements featuring up to 15 biomarkers. See how to characterize tissue microenvironments and examine the differences between normal and disease tissues without the need to code or train Deep Learning models—all with Aivia. Plus, get your 3D spatial biology results faster with an inbuilt, enhanced Deep Learning model that can accurately detect and partition cells with morphological variations. You will discover how to leverage your expertise or simple automation to classify cells into different phenotypes and interactively explore them in their spatial context. We also demonstrate how, with a few clicks, you can produce key measurements such as percentage distribution, Pearson correlation coefficient, and dimensionality reduction. You will also learn how to visualize the relationship between biomarkers and clusters using dendrograms.Watch the full presentation here: https://microscopyfocus.com/enhancing-3d-spatial-biology-with-ai-simplified-insights-for-all/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Accelerate Scientific Progress: The Power of Reproducibility, Collaboration and New Imaging Technologies

Accelerate Scientific Progress: The Power of Reproducibility, Collaboration and New Imaging Technologies

Imaging scientists have a critical educational role in improving rigor and reproducibility by sharing their technical expertise and providing intellectual contributions in all aspects of image-based science. They participate in creating, developing, and disseminating guidelines, resources, and tools to improve image-based research.In this episode, learn what impacts reproducibility in microscopy, discover resources and initiatives for improving education, rigor, and reproducibility, and see how collaboration drives innovation.Watch the full presentation here: https://microscopyfocus.com/accelerate-scientific-progress-the-power-of-reproducibility-collaboration-and-new-imaging-technologies/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Inside Immunoassays: Best Practices for Accurate and Reliable Results

Inside Immunoassays: Best Practices for Accurate and Reliable Results

Immunoassays play an indispensable role in scientific research. They facilitate the detection and quantification of specific analytes, enabling in-depth investigations into disease mechanisms, biomarker discovery, and evaluation of the potential of therapeutic interventions.Sample selection and preparation are of utmost importance, as they have a direct impact on the accuracy, reliability, and integrity of the obtained results.Properly selecting and preparing samples ensures that they are representative of the population under study and that the analytes of interest are preserved in their native state.In addition, controlling and mitigating matrix interference is crucial in immunoassays to minimize the influence of sample components that may hinder the accuracy of measurements.In this episode, hear from immunoassay experts as they guide you through all these areas. Watch the full presentation here: https://events.bitesizebio.com/inside-immunoassays-best-practices/joinBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Windows On Neurovascular Pathologies

Windows On Neurovascular Pathologies

The brain is an immune-privileged organ, meaning it is protected from the entry of pathogens, blood circulating factors, and immune cells by a physical barrier called the blood-brain barrier (BBB). But pathological events such as ischemia (stroke), trauma (traumatic brain injury), or tumor growth (gliomas and brain metastasis), activate the vascular endothelium, disturb or disrupt the BBB and allow immune cell homing.In this episode, discover how innate immunity can sustain deleterious effects following brain trauma, and the technological developments enabling longitudinal studies into post-trauma lesion remodeling.Watch the full presentation here: https://microscopyfocus.com/windows-on-neurovascular-pathologies/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Unleashing Fibrotic Secrets: Coupling the Power of Laser Microdissection with Mass Spec

Unleashing Fibrotic Secrets: Coupling the Power of Laser Microdissection with Mass Spec

Tissue heterogeneity is a significant challenge when analyzing proteins in tissue samples to identify disease states. In this episode, explore how combining laser microdissection with mass spectrometry (LCM-MS) on fixed and stained tissues lets you explore the protein networks within pathological features to shed light on their molecular functions.Using hallmark lesions of lung fibrosis in the honeycomb airways and fibroblastic foci as an example, you'll hear how this technology can detect over 4,900 proteins from tissue volumes as low as 0.005 mm3.Watch the full presentation here: https://events.bitesizebio.com/unleashing-fibrotic-secrets/joinBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Virtual Reality Showcase for Stellaris: Leica’s Confocal Microscopy Platform

Virtual Reality Showcase for Stellaris: Leica’s Confocal Microscopy Platform

Join Advanced Workflow Specialists for a virtual reality showcase of Leica's confocal microscopy platform, STELLARIS. In this episode of Listen In, discover how to enhance your confocal imaging from performing multicolor experiments with clear, sharp images to capturing fluorescence intensity and lifetime-based information simultaneously.Watch the full presentation here: https://microscopyfocus.com/confocal-microscopy-stellaris-showcase/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Tools & Techniques for the Study of Biologics & Antibody Drug Conjugates

Tools & Techniques for the Study of Biologics & Antibody Drug Conjugates

The fundamental cellular mechanisms of internalization and trafficking are crucial to many areas of cell biology, especially the proper function of therapeutic antibodies. Antibodies intended for use as antibody-drug conjugates (ADCs) should specifically bind to target cells and rapidly internalize via endocytosis. However, the ability to study these processes has historically been limited by the lack of tools that directly monitor the internalization and subsequent catabolism of extracellular material. In this episode, get a demo of rapid, high-throughput methods to screen for antibody internalization. You will also hear about techniques to directly visualize the internalization of therapeutic antibodies and ADCs in live cells for structure-activity relationship studies. You will also learn how the insights provided by these approaches have the potential to accelerate biotherapeutic lead generation and development.Watch the full presentation here: https://events.bitesizebio.com/tools-techniques-for-the-study-of/joinBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Multiplex Annotated Tissue Imaging System (MANTIS®)

Multiplex Annotated Tissue Imaging System (MANTIS®)

Join us on Listen In as we unveil a breakthrough method for imaging skin immune cells, crucial for accurate diagnoses and personalized treatments. Traditional imaging faces challenges like high tissue autofluorescence and spectral spillover. However, our latest episode introduces a game-changing solution using the Leica SP8 microscope coupled with the MANTIS system.Learn how this innovative approach allows for the acquisition of up to 10 colors, providing detailed immune phenotyping of skin samples. Plus, discover how MANTIS simplifies complex imaging, making advanced analysis accessible and practical for routine clinical practice.Tune in to explore how this new technology can revolutionize the identification of skin immune cells and enhance diagnostic accuracy.Watch the full presentation here: https://microscopyfocus.com/multiplex-annotated-tissue-imaging/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Breaking Out of The Mold - A Panel Discussion of Alternative Careers

Breaking Out of The Mold - A Panel Discussion of Alternative Careers

Join us on Listen In for an inspiring episode that explores alternative careers for scientists who want to combine their artistic skills with their passion for science. If you love science but feel that traditional lab life isn’t for you, this episode is a must-listen.In this episode, we're joined by a panel of individuals who have carved out unique careers that merge science with creativity. Our panelists include a Marketing Communication Manager/Graphic Designer, an Artist in Residence, and a Science Rapper. These trailblazers will share their journeys, discuss how they found their unique paths, and provide insights into their innovative careers.Learn about the various opportunities available beyond the conventional academic and industry roles, and get inspired by the possibilities of combining science with art. Tune in to discover how you can turn your passion for science and art into a fulfilling career.Watch the full presentation here: https://events.bitesizebio.com/breaking-out-of-the-mold-a-panel-1/joinBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Five-Color STED With a Single Depletion Laser and Fluorescence Lifetime Phasor Separation

Five-Color STED With a Single Depletion Laser and Fluorescence Lifetime Phasor Separation

Dive into the innovative world of STED microscopy and explore how researchers are pushing the boundaries of super-resolution imaging in this episode of Listen In. Traditional STED microscopy achieves high resolution by using a doughnut-shaped depletion laser to selectively suppress fluorescence, but multicolor experiments often face challenges such as laser misalignment and dye bleaching.Explore the game-changing technique of using a single depletion wavelength combined with phasor-based FLIM separation. This method allows scientists to distinguish multiple fluorophores with similar emission spectra but different fluorescent lifetimes, significantly increasing the number of usable spectral channels.Discover how researchers successfully depleted five fluorochromes across different channels using a single laser and utilized the phasor plot for quick and easy visualization of staining quality and lifetime analysis. This approach not only doubles the number of distinguishable colors in laser scanning microscopy but also simplifies the process, making it accessible to many scientists with minimal training.Watch the full presentation here: https://microscopyfocus.com/five-color-sted/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Grant Writing: Everything but the Science

Grant Writing: Everything but the Science

Dive into the often daunting world of grant applications. Securing funding is critical for any researcher, but the process can be filled with tricky tasks and complex requirements. Join us as we explore strategies to handle common challenges in grant applications with expert advice.Learn how to tackle issues like:• Career disruption• Reason for choosing your place of work• Track record relative to opportunity• Consumer involvementThis episode guides you through the rationale behind these additional requirements, offers tips on how to effectively showcase your track record and collaborators, and provides insights on managing challenging grant situations. Don’t miss this essential advice that could make all the difference in securing your next grant!Watch the full presentation here: https://events.bitesizebio.com/grant-writing-everything-but-the-1/joinBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com

Live-cell Fluorescence Lifetime Multiplexing Using Organic Fluorophores

Live-cell Fluorescence Lifetime Multiplexing Using Organic Fluorophores

In this episode, we explore the cutting-edge techniques revolutionizing fluorescence microscopy. Traditional methods are limited to detecting three to four targets due to the spectral overlap of fluorophores. But now, scientists are breaking these barriers with fluorescence lifetime multiplexing. This innovative approach uses differences in fluorescence lifetimes to distinguish between similar dyes, allowing for more complex imaging.Hear how researchers combined lifetime multiplexing with spectral detection to analyze multiple targets simultaneously. Discover the exciting advancements made by characterizing 18 synthetic fluorophores and developing unique self-labeling protein tags. These breakthroughs enable the imaging of up to eight targets in four spectral channels, providing unprecedented detail in cellular studies.Watch the full presentation here: https://microscopyfocus.com/live-cell-fluorescence-lifetime-multiplexing-using-organic-fluorophores/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Stress in the Lab. Common Pitfalls and How to Avoid Them

Stress in the Lab. Common Pitfalls and How to Avoid Them

Stress is a universal challenge, and scientists face unique pressures such as impostor syndrome, the demand for positive results, and career uncertainty. These stressors can be overwhelming and often feel beyond our control.In this episode of Listen In, we explore the various stresses that scientists encounter in their daily lab life and discuss effective strategies to manage them. Learn how to prevent burnout, anxiety, and depression by addressing issues like perfectionism and impostor syndrome. Our expert shares personal insights and practical advice on cultivating a resilient and healthier growth mindset.Hit play to discover that you're not alone in feeling overwhelmed and that with the right mindset, you can navigate the pressures of a scientific career more effectively.Watch the full presentation here: https://events.bitesizebio.com/stress-in-the-lab-common-pitfalls-1/joinBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Visualizing Protein-Protein Interactions by Non-fitting and Easy FRET-FLIM Approaches

Visualizing Protein-Protein Interactions by Non-fitting and Easy FRET-FLIM Approaches

Understanding molecular interactions in living cells is one of the key elements to deciphering the molecular mechanisms underpinning most cellular functions. Förster resonance energy transfer (FRET) is the gold standard for studying protein-protein interactions.Fluorescence lifetime imaging microscopy (FLIM) allows for a straightforward quantification of FRET based on the behavior of donor-only fluorescence.Join Dr. Padilla-Parra in this episode of Listen In to learn about some of the limitations of classical FRET approaches, how much information you can harness from FRET, and how lifetime-based non-fitting approaches such as minimal fraction of interacting donors (mFD) can provide a direct readout of protein-protein interactions in the cellular environment over time.Watch the full presentation here: https://microscopyfocus.com/visualizing-protein-protein-interactions-by-non-fitting-and-easy-fret-flim-approaches/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com

Optimizing Nuclei Extraction & Counting for Single Cell Sequencing

Optimizing Nuclei Extraction & Counting for Single Cell Sequencing

Single cell sequencing samples must be prepared to the highest possible standard.Discover reliable techniques to prepare and validate nuclei samples for single cell sequencing. In this episode, learn how to perform essential quality control checks, including a quick and reliable method for quantifying nuclei, measuring extraction efficiency, and visualizing debris in a sample. You will also get tips to optimize your existing nuclei extraction protocols from an experienced Application Scientist.Watch the full presentation here: https://events.bitesizebio.com/optimizing-nuclei-extraction/joinBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

How to Effectively Communicate Your Research

How to Effectively Communicate Your Research

Join Kate Christian as she demystifies the process of effectively communicating your research in the latest episode of Listen In. She describes the impact of research communication in and out of the lab and provides some guides to explaining your research in various contexts, such as lab tours, community visits, and other public engagements.As well as learning how to work out your h-index, you will learn how to describe the impact of your papers.Watch the full presentation here: https://events.bitesizebio.com/how-to-effectively-communicate-your-1/joinBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Imaging GPCR Activity: Unravelling Mechanisms from Molecules to Morphology

Imaging GPCR Activity: Unravelling Mechanisms from Molecules to Morphology

Precise control of cellular communication and signaling is crucial for every physiological system, with G protein-coupled receptors (GPCRs, the largest family of signaling receptors) playing a central role as essential molecular sensors of a cell’s environment. Because of their diverse physiological and pathophysiological roles, GPCRs are also major drug targets. Our understanding of GPCR signaling has evolved from single receptors at the cell surface activating specific heterotrimeric G protein pathways, to an increasingly complex receptor signaling system. However, the complexity of GPCR signaling is translated to specific cellular and physiological responses is still unclear.In this episode, we explore some of the latest endeavors to uncover GPCR signaling mechanisms through a wide range of imaging applications, from analyzing single molecules to the identification of new subcellular signal platforms.Watch the full presentation here: https://microscopyfocus.com/imaging-gpcr-activity-unravelling-mechanisms-from-molecules-to-morphology/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Critical Analysis Of Research Papers: Practical Examples

Critical Analysis Of Research Papers: Practical Examples

As a researcher, one of the most crucial skills to develop is the ability to perform an efficient yet thorough review of manuscripts. Manuscript reviewing requires specific skills and knowledge to properly assess published literature. By reading and reviewing papers, researchers can develop and hone these skills, which will help them identify and handle tricky issues such as image and data manipulation.In this episode of Listen In, Dr. Johanna Ahlskog discusses the essential skills all reviewers and researchers need, emphasizes the critical importance of the review process, and offers general considerations when reviewing articles.Watch the full presentation here: https://events.bitesizebio.com/critical-analysis-of-research-1/joinBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

New TauSTED Tools for Gentle Live Imaging at Nanoscale Resolution

New TauSTED Tools for Gentle Live Imaging at Nanoscale Resolution

This episode of Listen In showcases the latest tools and developments for multiplex STED imaging of living specimens at nanoscale resolution. Confocal imaging is a fundamental tool for studying the complex interplay of biomolecules, molecular machines, and higher-order cellular structures owing to its optical sectioning, sensitivity, and temporal and spatial resolution capabilities.Imaging complex cellular structures at nanoscale resolution while characterizing the dynamics of multiple species in live specimens are emerging avenues to shed light on biological processes in their native physiological context.With the advent of STED, researchers have realized the visualization of intracellular structures at the nanoscale. Tune in to see how STED is enabling unprecedented insights into cellular behavior, interactions, and function.Watch the full presentation here: https://microscopyfocus.com/new-tausted-tools-for-gentle-live-imaging-at-nanoscale-resolution/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/